Studies on phospholipids. 7. The distribution of complex phospholipids in various species and tissues*

Physical Studies of Phospholipids

On heating pure, fully saturated 2,3-diacyl-DL-phosphatidyl-ethanolamines and 2,3-diacylphosphatidyl-cholines (lecithins) in water to the transition temperature at which large endothermic heat changes occur, they are observed, by light microscopy, to form myelin figures. This result is discussed in terms of the large difference in the transition temperature for "melting" of the hydrocarbon chai...

Studies on the role of phospholipids in phagocytosis.

Separation of phospholipids and individual molecular species of phospholipids by high-performance liquid chromatography.

Isocratic high-performance liquid chromatography methods are described for separating the major classes of phospholipids and for isolating the individual molecular species of phospholipids. Fractionation of a total lipid extract of rat liver on a silica column resulted in quantitative recoveries of all major phospholipids with preservation of their fatty acid composition. Rat liver phosphatidyl...

Physical studies of phospholipids. IV. High resolution nuclear magnetic resonance spectra of phospholipids and related substances.

The nuclear magnetic resonance spectra of phosphatidylethanolamines, phosphatidylcholines, phosphatidylserines, and sphingomyelin are discussed in terms of molecular structure. The factors influencing the band width of the protons of the polar head of phosphatidylcholines are surveyed. It is found that the band widths vary with solvent and that this variation can perhaps be correlated with the ...

Studies on Phospholipids. 9. the Composition of Rat-liver Lecithins.